5′-3′ RNA-RNA Interaction Facilitates Cap- and Poly(A) Tail-independent Translation of Tomato Bushy Stunt Virus mRNA

On the rigidity of RNA in tomato bushy stunt virus.

The motional state of RNA in tomato bushy stunt rirlls, both in thr crystalline st,attx and in solution, has been invrstigat,ed IuGng 31P nuclear magnetic resonance methods. It, has been found that t’hr RNA is highly immobile in tho nativ Crus. and it is suggested that t,he lack of a high-resolution X-ray diffraction patt,crn for either the RNB or the N-terminal repions of the pro&n coat molcrn...

Tomato bushy stunt virus co-opts the RNA-binding function of a host metabolic enzyme for viral genomic RNA synthesis.

Tomato bushy stunt virus (TBSV), a plus-stranded [(+)] RNA plant virus, incorporates the host metabolic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH) into the viral replicase complex. Here, we show that, during TBSV replication in yeast, the yeast GAPDH Tdh2p moves from the cytosol to the peroxisomal membrane surface, the site of viral RNA synthesis. In yeast cells lacking Tdh2p, decr...

Purification of Tomato Bushy Stunt Virus by Differential Centrifugation

Physicochemical Studies on Tomato Bushy Stunt Virus Variants

Tomato bushy stunt virus particles have previously been physically and chemically well characterized. Different strains, however, are known to have different host plants and symptoms, and they can be distinguished serologically. In an attempt to correlate this behavior with physicochemical properties, we have performed a comparative study on isolates of four of the main variants of this virus (...

Tomato bushy stunt virus (TBSV) infecting Lycopersicon esculentum.

Tomato bushy stunt virus (TBSV) was detected in tomato crop (Lycopersicon esculentum) in Egypt with characteristic mosaic leaf deformation, stunting, and bushy growth symptoms. TBSV infection was confirmed serologically by ELISA and calculated incidence was 25.5%. Basic physicochemical properties of a purified TBSV Egh isolate were identical to known properties of tombusviruses of isometric 30-...